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人抗中性粒細(xì)胞顆??贵w酶免試劑盒,(ANGA)ELISA檢測試劑盒,英文名:ANGA ELISA Kit,英文縮寫:ANGA ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗中性粒細(xì)胞顆??贵wELISA檢測試劑盒,ANGA 檢測試劑盒,ANGA 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人抗中性粒細(xì)胞顆粒抗體酶免試劑盒,(ANGA)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重
紅細(xì)胞生成素(EPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Erythropoietin (EPO)48T/96TY08385Homo sapiens (Human)
紅細(xì)胞生成素(EPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Erythropoietin (EPO)48T/96TY08386Mus musculus (Mouse)
E選擇素(SELE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Selectin, Endothelium (SELE)48T/96TY08387Homo sapiens (Human)
E選擇素(SELE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Selectin, Endothelium (SELE)48T/96TY08388Mus musculus (Mouse)
凋亡相關(guān)因子(FAS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Factor Related Apoptosis (FAS)48T/96TY08389Homo sapiens (Human)
凋亡相關(guān)因子(FAS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Factor Related Apoptosis (FAS)48T/96TY08390Mus musculus (Mouse)
凋亡相關(guān)因子配體(FASL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Factor Related Apoptosis Ligand (FASL)48T/96TY08391Homo sapiens (Human)
凋亡相關(guān)因子配體(FASL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Factor Related Apoptosis Ligand (FASL)48T/96TY08392Mus musculus (Mouse)
酸性成纖維細(xì)胞生長因子(FGF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1)48T/96TY08393Homo sapiens (Human)
成纖維細(xì)胞生長因子4(FGF4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 4 (FGF4)48T/96TY08394Homo sapiens (Human)
粒細(xì)胞巨噬細(xì)胞集落刺激因子(GMCSF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)48T/96TY08395Rattus norvegicus (Rat)
糖蛋白130(gp130)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucoprotein 130 (gp130)48T/96TY08396Homo sapiens (Human)
糖蛋白130(gp130)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucoprotein 130 (gp130)48T/96TY08397Mus musculus (Mouse)
肝細(xì)胞生長因子(HGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hepatocyte Growth Factor (HGF)48T/96TY08398Homo sapiens (Human)
細(xì)胞間粘附分子1(ICAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1)48T/96TY08399Homo sapiens (Human)
細(xì)胞間粘附分子1(ICAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1)48T/96TY08400Mus musculus (Mouse)
細(xì)胞間粘附分子1(ICAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1)48T/96TY08401Rattus norvegicus (Rat)
干擾素γ(IFNγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY08402Homo sapiens (Human)
輔肌動蛋白α2(ACTN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Actinin Alpha 2 (ACTN2)48T/96TY08403Rattus norvegicus (Rat)
輔肌動蛋白α2(ACTN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Actinin Alpha 2 (ACTN2)48T/96TY08404Mus musculus (Mouse)
血小板激活因子(PAF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Activating Factor (PAF)48T/96TY08405Mus musculus (Mouse)
凝血因子Ⅵ(F6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor VI (F6)48T/96TY08406Homo sapiens (Human)
纖維蛋白原(FG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinogen (FG)48T/96TY08407Rattus norvegicus (Rat)
纖維蛋白原(FG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinogen (FG)48T/96TY08408Mus musculus (Mouse)
生長抑素(SST)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Somatostatin (SST)48T/96TY08409Homo sapiens (Human)
生長抑素(SST)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Somatostatin (SST)48T/96TY08410Rattus norvegicus (Rat)
生長抑素(SST)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Somatostatin (SST)48T/96TY08411Mus musculus (Mouse)
β-內(nèi)啡肽(βEP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-Endorphin (bEP)48T/96TY08412Rattus norvegicus (Rat)
胃動素(MTL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Motilin (MTL)48T/96TY08413Homo sapiens (Human)
胃動素(MTL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Motilin (MTL)48T/96TY08414Oryctolagus cuniculus (Rabbit)
降鈣素基因相關(guān)肽(CGRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calcitonin Gene Related Peptide (CGRP)48T/96TY08415Homo sapiens (Human)
降鈣素基因相關(guān)肽(CGRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calcitonin Gene Related Peptide (CGRP)48T/96TY08416Rattus norvegicus (Rat)
降鈣素基因相關(guān)肽(CGRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calcitonin Gene Related Peptide (CGRP)48T/96TY08417Mus musculus (Mouse)
碳酸酐酶Ⅰ(CA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carbonic Anhydrase I (CA1)48T/96TY08418Homo sapiens (Human)
碳酸酐酶Ⅰ(CA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carbonic Anhydrase I (CA1)48T/96TY08419Rattus norvegicus (Rat)
碳酸酐酶Ⅰ(CA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carbonic Anhydrase I (CA1)48T/96TY08420Mus musculus (Mouse)
內(nèi)皮脂肪酶(LIPG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipase, Endothelial (LIPG)48T/96TY08421Homo sapiens (Human)
內(nèi)皮脂肪酶(LIPG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipase, Endothelial (LIPG)48T/96TY08422Rattus norvegicus (Rat)
內(nèi)皮脂肪酶(LIPG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipase, Endothelial (LIPG)48T/96TY08423Mus musculus (Mouse)
妊娠關(guān)聯(lián)血漿蛋白A(PAPPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pregnancy Associated Plasma Protein A (PAPPA)48T/96TY08424Homo sapiens (Human)
妊娠關(guān)聯(lián)血漿蛋白A(PAPPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pregnancy Associated Plasma Protein A (PAPPA)48T/96TY08425Rattus norvegicus (Rat)
妊娠關(guān)聯(lián)血漿蛋白A(PAPPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pregnancy Associated Plasma Protein A (PAPPA)48T/96TY08426Mus musculus (Mouse)
高遷移率族蛋白1(HMG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for High Mobility Group Protein 1 (HMG1)48T/96TY08427Homo sapiens (Human)
高遷移率族蛋白1(HMG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for High Mobility Group Protein 1 (HMG1)48T/96TY08428Rattus norvegicus (Rat)
高遷移率族蛋白1(HMG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for High Mobility Group Protein 1 (HMG1)48T/96TY08429Mus musculus (Mouse)
脫氫表雄酮(DHEA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dehydroepiandrosterone (DHEA)48T/96TY08430General
轉(zhuǎn)化生長因子β受體Ⅰ(TGFβR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta Receptor I (TGFbR1)48T/96TY08431Homo sapiens (Human)
轉(zhuǎn)化生長因子β受體Ⅰ(TGFβR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta Receptor I (TGFbR1)48T/96TY08432Rattus norvegicus (Rat)
轉(zhuǎn)化生長因子β受體Ⅰ(TGFβR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta Receptor I (TGFbR1)48T/96TY08433Mus musculus (Mouse)
性激素結(jié)合球蛋白(SHBG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sex Hormone Binding Globulin (SHBG)48T/96TY08434Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。